Proteomics study reveals the molecular mechanisms underlying cryotolerance induced by mild sublethal stress in human sperm

Autor: Hossein Baharvand, Yunqi Wu, Mohsen Sharafi, Vivek Gupta, Matthew J. Fitzhenry, Homa Mohseni Kouchesfahani, Mehdi Mirzaei, Paul A. Haynes, A Dalman, Poopak Eftekhari-Yazdi, Abdolhossein Shahverdi, Ghasem Hosseini Salekdeh, Maryam Hezavehei
Rok vydání: 2021
Předmět:
Zdroj: Cell and Tissue Research. 387:143-157
ISSN: 1432-0878
0302-766X
DOI: 10.1007/s00441-021-03537-1
Popis: The preconditioning of human sperm with sublethal nitrosative stress before cryopreservation can potentially improve the thawed sperm quality. However, the underlying mechanisms behind this protective strategy are not entirely understood. We compared the cryosurvival of human sperm exposed to 0.01 μM nitric oxide (NO) throughout the cryopreservation and used multiplexed quantitative proteomics approach to identify changes in the proteome profile of preconditioned sperm cells. Semen samples were obtained from 30 normospermia donors and then each sample was divided into three equal parts: fresh (F), frozen-control (C), and frozen exposed to nitric oxide (NO). The sperm undergoing mild sublethal stress showed higher values for motility and viability compared to the frozen control sperm. Moreover, out of 2912 identified proteins, 248 proteins were detected as differentially abundant proteins (DAPs) between cryopreserved groups and fresh group (F) (p
Databáze: OpenAIRE
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