| Autor: |
Jörg Pietruszka, N. M. Myasoedova, Elena Podieiablonskaia, Ludmila A. Golovleva, M. P. Kolomytseva, Thomas Classen, A.M. Chernykh, Anastasia Samoilova |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Process Biochemistry. 62:174-183 |
| ISSN: |
1359-5113 |
| Popis: |
During the last decade the search for novel biotechnologically valuable laccases/oxidases with a high redox potential and concomitant activity under neutral-alkaline conditions is an attractive and at the same time complicated task due to their rare occurrence in nature. By means of the modified micromethod based on the chromogenic reaction with indicator substrates the successful identification of laccases/oxidases with different pH-optimum was carried out during submerged cultivation of 71 fungal strains of different taxonomic groups. Based on more sensitivity (detected laccase activity can be 4–6 time less as compared with the usual spectrophotometric assay of laccase activity), good productivity (measurements of numerous samples at once in small total volume – up to 150 μL), economy and rapidity, the presented modification of chromogenic reaction can be applied for identification of trace amount of laccase/oxidase activity in biological liquids, to determine the chemoselectivity of induced laccase/oxidase isoforms with respect to pH-value of medium, and to monitor the dynamics of expression of alkaliphilic and acidophilic laccases/oxidases during submerged cultivation of fungi. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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