Development and In Vitro Characterization of a New Immunoassay of Cardiac Troponin T

Autor:	Andrew Remppis, A Borgya, U Essig, Klaus Hallermayer, U Geuss, T Scheffold, Hugo A. Katus, S Looser
Rok vydání:	1992
Předmět:	medicine.diagnostic_test Troponin T biology business.industry medicine.drug_class Biochemistry (medical) Clinical Biochemistry Monoclonal antibody Troponin Molecular biology Antigen Immunoassay Immunoblot Analysis medicine biology.protein Hybridoma technology Antibody business
Zdroj:	Clinical Chemistry. 38:386-393
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/38.3.386
Popis:	We describe a new one-step enzyme immunoassay of troponin T that uses two specific monoclonal antibodies and streptavidin-coated tubes as the solid phase. The monoclonal antibodies were obtained by conventional hybridoma technology, with human troponin T as antigen. The identity of the cardiac troponin T antigen was confirmed by analysis of the amino acid composition and by partial sequence analysis. The specificity of the monoclonal antibodies for cardiac troponin T was proved by immunoblot analysis and displacement curves. The capture antibody is labeled with biotin. The second antibody is conjugated to horseradish peroxidase (EC 1.11.1.7). The assay [Enzymun-TestR system (Boehringer Mannheim GmbH)] is performed in only 90 min at room temperature; the measuring range for troponin T is 0.1 to 15 micrograms/L. The assay shows excellent between-run precision (CV = 3.3-4.9%).
Databáze:	OpenAIRE
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