Liquid chromatographic and potentiometric methods for deteminations of clopidogrel
| Autor: | Alaa Alside Amin, H. M. Killa, Mohmed Alaa Elmosallamy, Amr L. Saber |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
0303 health sciences Chromatography 030309 nutrition & dietetics Formic acid 010401 analytical chemistry Potentiometric titration Phthalate Ether 01 natural sciences Diluent 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound chemistry Ammonium formate Acetonitrile Selectivity Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 16 |
| ISSN: | 2224-6614 |
| DOI: | 10.38212/2224-6614.2360 |
| Popis: | Two different techniques are developed for the determination of clopidogrel in pharmaceutical preparations. HPLC method has been developed where chromatographic analysis is performed on Nova-Pak(superscript ®) C18 column (3.9 mm×150 mm, 5 μm) with an ammonium formate buffer adjusted with formic acid to pH 4.0, acetonitrile (40:60, v/v) as mobile phase, and detection at 225 nm. Good linearity (0.9993, r), accuracy (≥99.20%) and precision (≤0.6 RSD) were obtained. Potentiometric measurments are based on tetrakis (p-chlorophenyl) borate-clopidogrel ion-pair as an electroactive material incorporating a plasticized PVC membrane with o-nitrophenyl octyl ether or dioctyl phthalate. The sensor is conditioned for at least two days in 0.1 M drug solution before use. It exhibits fast and stable Nernstian response for clopidogrel over the concentration range of 1.0×10^(-5)~1.0×10^(-2) M and pH range of 1.5-4.0. Results with an average recovery of 100.6% and a mean standard deviation of 0.86% of the nominal were obtained. The sensor shows reasonable selectivity towards clopidogrel hydrogen sulphate in presence of many cations. No significant interferences are caused by drug excipients and diluents. |
| Databáze: | OpenAIRE |
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