Process Development for Biocatalytic Oxidations Applying Alcohol Dehydrogenases

Autor:	Theo Sonke, Jan Brummund, Monika Müller
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Oxidase test biology Chemistry Organic Chemistry Alcohol Cofactor chemistry.chemical_compound Glycerol-3-phosphate dehydrogenase Enzyme biology.protein Organic chemistry NAD+ kinase Physical and Theoretical Chemistry Acetophenone Alcohol dehydrogenase
Zdroj:	Organic Process Research & Development. 19:1590-1595
ISSN:	1520-586X 1083-6160
Popis:	Alcohol dehydrogenases are able to catalyze the conversion of alcohols to aldehydes or ketones, simultaneously reducing the cofactor NAD+ or NADP+ to NAD(P)H. Because of the high costs of these pyridine cofactors, in situ cofactor regeneration is required for preparative applications in order to reach turnover numbers that are sufficient for economically viable processes. Here we present the development of a process for the enantioselective oxidation of rac-1-phenylethanol to acetophenone, applying an alcohol dehydrogenase coupled with an NAD(P)H oxidase for the enzymatic cofactor regeneration, which is active towards NADH as well as NADPH. The reaction system was investigated in view of various influential parameters with main focus on the external oxygen supply. We could show that a gassed stirred tank reactor is a promising reactor concept to run NAD(P)H oxidase-coupled alcohol dehydrogenase oxidations, including the possibility to scale-up the system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dcd600a3aef5f31c5999a2670ac6d255 https://doi.org/10.1021/op500307e Zobrazit plný text záznamu