Novobiocin blocks the shutoff of SP01 early transcription

Autor:	Alberto N. Sarachu, Oscar Grad, Juan C. Alonso
Rok vydání:	1980
Předmět:	Genetics General transcription factor DNA replication Promoter Biology biology.organism_classification DNA gyrase Bacteriophage chemistry.chemical_compound chemistry Transcription (biology) Virology Gene DNA
Zdroj:	Virology. 105:13-18
ISSN:	0042-6822
DOI:	10.1016/0042-6822(80)90151-8
Popis:	Novobiocin, an inhibitor of bacterial DNA gyrase strongly impairs the development of bacteriophage SPOl. DNA replication seems to be the primary target for the antibiotic in this system, but viral-coded transcription is also affected in several aspects: (a) The level of phage transcription is diminished; (b) the shutoff of the synthesis of early RNA classes is inhibited; (c) RNAs of late class are not synthesized. This last effect is a consequence of the coupling between transcription and replication. The other two results could be taken as an indication that the appropriate secondary structure of the parental phage DNA is a requisite for the recognition of promoters. The introduction of negative turns by DNA gyrase seems to make early genes unavailable for transcription.
Databáze:	OpenAIRE
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