One-Step, Labeled-Antibody Assay for Measuring Free Thyroxin. I. Assay Development and Validation

Autor:	J E M Midgley, C P Sheehan, N D Christofides
Rok vydání:	1992
Předmět:	Biochemistry (medical) Clinical Biochemistry hormones hormone substitutes and hormone antagonists
Zdroj:	Clinical Chemistry. 38:11-18
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/38.1.11
Popis:	We describe a one-step, labeled-antibody radioassay for measuring free thyroxin (FT4) in serum or plasma, based on a novel principle. FT4 in the sample competes with a gross molar excess (over antibody) of a cross-reactant (L-triiodothyronine, T3), chemically coupled to magnetizable polymer particles, for binding to avid 125l-labeled monoclonal anti-thyroxin antibodies. As in conventional immunoassays, 125I counts bound to the solid phase (T3-magnetizable particles) are inversely proportional to sample FT4 concentration. We demonstrate here the development and technical validity of this new method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dcd18f9b1417a7fe6dc47a187b148575 https://doi.org/10.1093/clinchem/38.1.11 Zobrazit plný text záznamu