HiFi-SIM: reconstructing high-fidelity structured illumination microscope images
| Autor: | Hui Li, Yuguo Tang, Liang Yong, Simin Li, Wang Linbo, Gang Wen, Chen Xiaohu, Zhenglong Sun, Jin Xin |
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| Rok vydání: | 2020 |
| Předmět: |
Point spread function
Ideal (set theory) Microscope business.industry Fluorescence microscopy technique Computer science media_common.quotation_subject Structured illumination microscopy Fidelity Reconstruction algorithm Structured illumination law.invention Optics High fidelity law Calibration business media_common |
| Popis: | Structured illumination microscopy (SIM) has been a widely-used super-resolution (SR) fluorescence microscopy technique, but artifacts often appear in reconstructed SR images which reduce its fidelity and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly-seen artifacts without loss of fine structures and improve the axial sectioning. Since results of HiFi-SIM are not sensitive to used PSF and reconstruction parameters, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool. |
| Databáze: | OpenAIRE |
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