Comparative analysis of gut microbiota of breast‐ and formula‐fed Korean infants by using pyrosequencing (1017.4)

Autor:	Ji Ye Lim, Yuri Kim, Mi Na Rhie, Ok Bin Kim, Sang A Lee, Bongsoo Kim, Su Jin Cho
Rok vydání:	2014
Předmět:	education.field_of_study biology Population Gut flora Breast milk biology.organism_classification Biochemistry Microbiology Bifidobacteriaceae fluids and secretions Genetics Pyrosequencing Microbiome education Molecular Biology Bacteria Feces Biotechnology
Zdroj:	The FASEB Journal. 28
ISSN:	1530-6860 0892-6638
DOI:	10.1096/fasebj.28.1_supplement.1017.4
Popis:	Infant feeding is one of the significant determinants of the intestinal microbiota in early life. Although numerous researches have performed to investigate the bacteria community in the infant gut, culture-based methods had showed limited bacteria population. Recently, pyrosequencing, a new high throughtput DNA sequencing technique, has been introduced and applied to explore microbiome. The purpose of this study was to compare the compositions of the fecal microbiota of Korean infants fed breast milk to those of infants fed milk formula. We examined microbial communities in fecal samples collected from 20 Korean infants at 4 weeks of age using pyrosequencing. Diversity analysis of total microbiota sequences revealed that a more diverse collection in milk formula-fed infants (MFI), whereas, the probiotics, including S. salivarius, L. gasseri, and B. longum were abundant in the microbiota of breast milk-fed infants (BFI). Moreover, Bifidobacteriaceae were significantly abundant in BFI compared with those o...
Databáze:	OpenAIRE
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