Cell Differentiation Agent-2 (CDA-2) Inhibits the Growth and Migration of Saos-2 Cells via miR-124/MAPK1
| Autor: | Youke Xie, Changyi Liu, Na Chen, Quanxiu Li, Guangchun Li, Bangyu Deng |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gene knockdown Chemistry Cell growth Cellular differentiation Cell migration medicine.disease Molecular biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis Cancer cell medicine Osteosarcoma MTT assay Saos-2 cells |
| Zdroj: | Cancer Management and Research. 12:4541-4548 |
| ISSN: | 1179-1322 |
| DOI: | 10.2147/cmar.s248851 |
| Popis: | Background CDA-2 (cell differentiation agent 2), isolated from healthy human urine, exerts antitumor effects in multiple types of cancer cells. However, its role in osteosarcoma has not been studied. Methods The MTT assay was used to examine the cell proliferation rate. A colony formation assay was used to examine cell growth. The Transwell assay was used to examine cell migration ability. A real-time PCR assay was used to examine the expression levels of miR-124 and MAPK1. A Western blot assay was used to examine protein expression levels. MAPK1 was selected as a possible target of miR-124, and the targeting relationship was examined by a luciferase reporter assay. Results We revealed that CDA-2 decreased the growth, migration and invasion ability of the osteosarcoma cell line Saos-2. Further study revealed that CDA-2 elevated the expression level of miR-124. MAPK1 was identified as a downstream target of miR-124. Knockdown of miR-124 or overexpression of MAPK1 counteracted CDA-2's effects on cell growth and invasion. Conclusion Our data revealed that the miR-124/MAPK1 axis mediated CDA-2's function in Saos-2 cells. CDA-2 can be used as a new treatment strategy for osteosarcoma. |
| Databáze: | OpenAIRE |
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