The Effect of Monoclonal Anti-human-platelet Antibodies on Platelet Kinetics in a Baboon Model: IgG Subclass Dependency

Autor:	L M Coetzee, J. P. Roodt, S De Reys, S Cooper, H. Pieters, V. Van Wyk, Philip N. Badenhorst
Rok vydání:	2000
Předmět:	medicine.diagnostic_test biology business.industry Hematology medicine.disease Immunoglobulin E Bleeding time In vivo Immunology Monoclonal biology.protein Medicine IgG deficiency Platelet Platelet activation Antibody business
Zdroj:	Thrombosis and Haemostasis. 83:148-156
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0037-1613771
Popis:	SummaryWe assessed the in vivo effect of six intact anti-human antiplatelet antibodies of two major IgG subclasses on platelet kinetics in baboons. Five of the six antibodies tested caused thrombocytopenia of varying degree when injected at a precalculated threshold value. An agglutinating IgG1 antibody (MA-8L4A12) caused a long-lasting, mild thrombocytopenia with a predominant uptake of radiolabelled platelets in the spleen, while the four IgG2 antibodies tested (MA-13G8E1, MA-2M5A6, MA-21K2E8 and MA-22M10) caused a severe, transient thrombocytopenia with uptake of platelets in the liver. Two of the IgG2 antibodies (MA-13G8E1 and MA-2M5A6) caused platelet activation and aggregation in vitro, whilst the other two did not elicit a platelet aggregation response. The platelet survival time was shortened with all five of the thrombocytopenia-inducing antibodies, while only one antibody (MA-2M5A6) had a significant effect on the bleeding time. This study indicates that the IgG subclasss may be a determining factor in the outcome of platelet sequestration in immune-induced thrombocytopenia.
Databáze:	OpenAIRE
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