Abstract 2956: c-FLIP (L) dual function in cervical cancer

Autor: Pamela Leal, Tamara Viscarra, Patricia García, Oscar Tapia, Juan Carlos Roa, Priscilla Brebi, Helga Weber, Carmen Ili, Alejandra Andana
Rok vydání: 2013
Předmět:
Zdroj: Cancer Research. 73:2956-2956
ISSN: 1538-7445
0008-5472
DOI: 10.1158/1538-7445.am2013-2956
Popis: Cellular FLICE-like inhibitory protein (c-FLIP) is a caspasa-8/10 homolog catalytically inactive that interferes with the efficient formation of DISC. There are three isoforms of this protein: c-FLIPL (long) of 55kDa, c-FLIPS (short) of 26kDa y c-FLIPR (Raji) of 24kDa. c-FLIPS and c-FLIPR functions have been well established: both can block extrinsic pathway of apoptosis by inhibiting procaspasa-8 activation in DISC. However, c-FLIPL function still remains unclear. When c-FLIPL is overexpressed, as in some cancers, has an anti-apoptotic function very similar to c-FLIPS, but also can be a proapoptotic molecule at low concentrations. The aim of this study is to characterize c-FLIP functions in cervical uterine carcinogenesis. Three cervical cancer cell lines were used in this study: SiHa, C-4I and C-33A. c-FLIP expression in cell lines was determined by real time PCR and western blottting. c-FLIP expression was transiently downregulated by siRNA and the silencing effects on cell viability, proliferation and apoptosis were analysed, by comparing with a control negative siRNA-transfected cells. Using MTS analysis, SiHa and C4I c-FLIP transfected cells showed a increment of viability compared with scramble, since 24 hours after transfection in C 4I and 72 hours in SiHa (P Our results suggest that c-FLIPL could be having a dual function in cervical cancer cell lines, both inhibiting proliferation and apoptosis. Grant Support: This investigation was financed by Proyect CORFO-INNOVA N°07CN13PBT-222 and Proyect CORFO N° 09CN14-5960 (CEGIN). CI is recipient of grants from FONDECYT Postdoctoral Proyect N° 3130630. PB is recipient of grants from FONDECYT Postdoctoral Proyect N° 3120141 Citation Format: Carmen G. Ili, Priscilla Brebi, Alejandra Andana, Patricia Garcia, Pamela Leal, Oscar Tapia, Tamara Viscarra, Helga Weber, Juan Roa. c-FLIP (L) dual function in cervical cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2956. doi:10.1158/1538-7445.AM2013-2956 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.
Databáze: OpenAIRE
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