Overexpression of RHO Inhibitor and PAK as Possible Treatments For Restoring Sharp Features in Cancer Cells

Autor:	Carol A. Heckman, V Sverdlov, J. A. Barnes, T S Wales, J. M. Urban, Louis Lim, C Hall, M. L. Cayer, R Kozma
Rok vydání:	2001
Předmět:	chemistry.chemical_compound Chemistry Cancer cell Second messenger system Phorbol CDC42 Protein kinase A Instrumentation Filopodia Phenotype Diacylglycerol kinase Cell biology
Zdroj:	Microscopy and Microanalysis. 7:576-577
ISSN:	1435-8115 1431-9276
DOI:	10.1017/s1431927600028956
Popis:	The mechanism of action of the tumor promoter, phorbol 12-myristate 13-acetate (PMA), depends on its ability to substitute for an endogenous second messenger, diacylglycerol, and thereby activate certain members of an enzyme family known as protein kinase C. Previous work from this laboratory showed that the quantitative shape phenotype of cells treated with PMA resembled the phenotype of bona fidecancer cells. The effect of PMA on this phenotype was transient, and was restricted to a period of two- to five-hours after exposure to PMA. When the shape phenotype was dissected into components by relating different variable's values to shape features, several of the altered values appeared to rely upon a declining number of sharp features, such as filopodia and microspikes, at the cell edge.Filopodia and microspikes are in turn regulated by a GTPase of the Rho family, Cdc42, which modulates actin architecture.
Databáze:	OpenAIRE
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