Changes in the pulmonary microbiome associated with lung cancer
| Autor: | Elisa Micholé, Ana Lucía Figueredo, Elisabet Vera, Rosa del Campo, Jose Javier Vengoechea, Manuel Ponce-Alonso, Antonio Rezusta, Javier Errasti, Salvador Bello, Laura Torralba |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Saliva biology Firmicutes medicine.drug_class business.industry Streptococcus Antibiotics respiratory system biology.organism_classification medicine.disease medicine.disease_cause Gastroenterology respiratory tract diseases Internal medicine medicine Oral Microbiome Microbiome Non small cell business Lung cancer |
| Zdroj: | Lung cancer. |
| DOI: | 10.1183/13993003.congress-2019.pa3663 |
| Popis: | Background: Despite the bacterial microbiota has been studied in the lung cancer context, the micobiome has not been explored yet. The aim of this study was to compare the bronchial and oral microbiome from patients with lung cancer with healthy controls Methods: A total of 25 lung cancer patients [non-small cell(18) and small cell lung cancer(7)], and 16 healthy controls were recruited. None had taken antibiotics in the previous month. Bronchial biopsies were obtained by broncoscopy from tumoral and contralateral non involved bronchi in cancer patients and from healthy bronchi in controls. Patients and controls also contributed with a saliva sample. Total DNA was obtained using the QiaAmp kit (Qiagen), determining the microbiome composition (16S rDNA V3-V4) and micobiome (ITS2) by massive sequencing in MiSeq platform (Illumina), statistical analysis in Qiime 2 and taxonomy assignation in the Silva19 database Results: Patients and controls had similar microbiome and micobiome in the saliva samples, which differ significantly from those obtained in the bronchial tract. The bronchial micobiome of patients and controls were also similar, whereas significant differences were observed for microbiota. The bronchi of lung cancer patients presented a higher bacterial charge (10^4vs10^3) than healthy controls, and a considerable increase of Firmicutes, mostly due to Streptococcus. Comparing the affected bronchi versus the contralateral, the differences were minor Conclusions: Despite the microbiome of saliva and bronchial tract were similar, ecological particularities of each anatomic localizations were observed. Micobiome seems not to be affected by the tumoral process, whereas the bacterial populations suffer several alterations in composition and density |
| Databáze: | OpenAIRE |
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