Laser induced resonance energy transfer — a novel approach towards achieving high sensitivity in capillary electrophoresis Part B: Applications for environmental testing
| Autor: | Anthony O. Okorodudu, Amin A. Mohammad, M. G. Bissell, John R. Petersen |
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| Rok vydání: | 1997 |
| Předmět: |
Detection limit
Aqueous solution Chromatography Organic Chemistry Analytical chemistry chemistry.chemical_element Terbium Ethylenediaminetetraacetic acid Ethylenediamine General Medicine Biochemistry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis chemistry Chelation Luminescence |
| Zdroj: | Journal of Chromatography A. 767:217-221 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(96)00799-6 |
| Popis: | Capillary electrophoresis with laser induced resonance energy transfer detection is used for direct determination of ethylenediamine tetraacetic acid (EDTA), 1,2-diaminopropane-N,N,N′,N′-tetraacetic acid (DPTA) and (±)- trans -1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (DCTA) in aqueous samples. The method involves CZE separation of salicylate-Tb-EDTA, salicylate-Tb-DPTA and salicylate-Tb-DCTA ternary complexes at alkaline pH. The highly efficient transfer of energy from salicylate to terbium in these complexes facilitated a sensitive detection. Detection limits in the 1·10 −7 M range are easily achieved. A 325 nm HeCd laser is used for excitation and terbium luminescence is monitored at 547 nm. The potential application of this method in analyzing these acids in a complex radioactive waste is discussed. |
| Databáze: | OpenAIRE |
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