In vitroAntiviral Activity of Ribavirin against Picornaviruses
| Autor: | James A. North, Roland K. Robins, Byron K. Murray, Douglas O. Andersen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
0301 basic medicine
viruses Ribavirin 030106 microbiology Picornaviridae virus diseases Biological activity General Medicine Biology medicine.disease_cause 01 natural sciences Virology Virus In vitro Poliovirus Type 1 0104 chemical sciences Microbiology 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound chemistry medicine Enterovirus Rhinovirus |
| Zdroj: | Antiviral Chemistry and Chemotherapy. 3:361-370 |
| ISSN: | 2040-2066 |
| DOI: | 10.1177/095632029200300606 |
| Popis: | The in vitro antiviral activity of ribavirin (Rby; 1-β-D-ribof uranosyl-1,2,4-triazole-3-carboxamide) against selected members of the Picornaviridae is described. When antiviral activity was determined by reduction of infectious virus progeny, Rbv was active against human rhinovirus type 2 (HRV-2) and poliovirus type 1 (Polio-1) in both a drug concentration and multiplicity of infection-(MOI) dependent response. However, an antiviral activity rating assay based on the reductions of cellular cytopathic effects (CPE) indicated that Rbv was active against human rhinoviruses, but was less active against polioviruses. Prophylactic administration of Rbv significantly improved virus yield reduction, especially in Polio-1. SDS Polyacrylamide gel electrophoresis (SDS-PAGE) of HRV-2- and Polio-1-infected cell lysates demonstrated that Rbv inhibited the synthesis of viral-specific proteins. Although actinomycin D (Act D) did not significantly influence Picornavirus yields, when added concomitantly with Rbv, Act D reversed Rbv's antiviral effect. |
| Databáze: | OpenAIRE |
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