Popis: |
In this discussion of in vitro perfusion of human placenta as an approach to the study of fetal nutrition I shall: (1) explain why, in recent years, we have discontinued using experimental animals and concentrated on human placenta; (2) describe the perfusion technique; (3) review our studies of two nutrients, glutamic acid and riboflavin, to illustrate the flexibility of experimental design that is possible and the type of information that can be obtained; (4) summarize the limitations and the potential of the perfusion technique as we have observed them. |