A novel method of immobilizing penicillin acylase on basic anion exchange resin with hexamethylene diisocyanate and p-hydroxybenzaldehyde
| Autor: | Bunji Kageyama, Takayasu Sonoyama, Shigeo Yagi, Kobee Kobayashi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Immobilized enzyme
biology Penicillin amidase biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound chemistry Reagent Yield (chemistry) Organic chemistry Hexamethylene diisocyanate Specific activity Ion-exchange resin Biotechnology Nuclear chemistry Bacillus megaterium |
| Zdroj: | Journal of Fermentation and Bioengineering. 74:410-412 |
| ISSN: | 0922-338X |
| DOI: | 10.1016/0922-338x(92)90044-u |
| Popis: | Penicillin acylase from Bacillus megaterium was immobilized to the functional groups of the basic anion exchange resin Diaion CR-20 using hexamethylene diisocyanate and p-hydroxybenzaldehyde as the coupling reagents. A specific activity of 135 U/g resin was obtained with an activity yield of 45%. The addition of 0.3 mM Ca2+ improved the stability of the immobilized enzyme, with its remaining activity in a recycling system at the 300th time being about 70% of the initial value. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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