Surface-functionalization of plasma-treated polystyrene by hyperbranched polymers and use in biological applications
| Autor: | Andrea Prager-Duschke, Alya Boulares-Pender, Michael R. Buchmeiser, Christian Elsner |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Polymers and Plastics biology Immobilized enzyme Chemical modification General Chemistry Branching (polymer chemistry) Surfaces Coatings and Films chemistry.chemical_compound chemistry Graft polymer Polymer chemistry Materials Chemistry biology.protein Surface modification Polystyrene Glutaraldehyde Bovine serum albumin |
| Zdroj: | Journal of Applied Polymer Science. 112:2701-2709 |
| ISSN: | 1097-4628 0021-8995 |
| Popis: | Nitrogen plasma was used to amino-func- tionalize polystyrene surfaces, which were further modi- fied via the selective introduction of polyamines suitable for the immobilization of biological compounds. This chemical modification was carried out using a multifunc- tional amine compound linked to glutaraldehyde, leading to the formation of hyperbranched structures at the sur- face. Up to three generations of branched polymers at the polystyrene (PS) surface were created by successive addi- tion of the functional compounds. Amine functions intro- duced at the surface were labeled with 2,3,4,5,6- pentafluorobenzaldehyde and analyzed by X-ray photo- electron spectroscopy (XPS), confirming the successful attachment of each generation of branching. Finally, bovine serum albumin and trypsin were immobilized on N2-plasma-treated PS modified with different amounts of branched graft polymer and found to remain bioactive after immobilization. V C 2009 Wiley Periodicals, Inc. J Appl |
| Databáze: | OpenAIRE |
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