Renal Overexpression of Atrial Natriuretic Peptide and Hypoxia Inducible Factor-1αas Adaptive Response to a High Salt Diet
| Autor: | Susana Gorzalczany, Elsa Zotta, Verónica Trida, Belisario E. Fernández, Andrea Carranza, María Inés Rosón, Jorge E. Toblli, Carolina S Cerrudo, Silvana Della Penna, Natalia Lucía Rukavina Mikusic, Alicia H. Correa, Gabriel Cao |
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| Rok vydání: | 2014 |
| Předmět: |
Kidney
medicine.medical_specialty General Immunology and Microbiology business.industry Kidney metabolism Renal function General Medicine Hypoxia (medical) medicine.disease_cause General Biochemistry Genetics and Molecular Biology Natriuresis medicine.anatomical_structure Endocrinology Atrial natriuretic peptide Internal medicine medicine Salt intake medicine.symptom business Oxidative stress |
| Zdroj: | BioMed Research International. 2014:1-10 |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/936978 |
| Popis: | In the kidney, a high salt intake favors oxidative stress and hypoxia and causes the development of fibrosis. Both atrial natriuretic peptide (ANP) and hypoxia inducible factor (HIF-1α) exert cytoprotective effects. We tested the hypothesis that renal expression of ANP and HIF-1αis involved in a mechanism responding to the oxidative stress produced in the kidneys of rats chronically fed a high sodium diet. Sprague-Dawley rats were fed with a normal salt (0.4% NaCl) (NS) or a high salt (8% NaCl) (HS) diet for 3 weeks, with or without the administration of tempol (T), an inhibitor of oxidative stress, in the drinking water. We measured the mean arterial pressure (MAP), glomerular filtration rate (GFR), and urinary sodium excretion (UVNa). We evaluated the expression of ANP, HIF-1α, and transforming growth factor (TGF-β1) in renal tissues by western blot and immunohistochemistry. The animals fed a high salt diet showed increased MAP andUVNalevels and enhanced renal immunostaining of ANP, HIF-1α, and TGF-β1. The administration of tempol together with the sodium overload increased the natriuresis further and prevented the elevation of blood pressure and the increased expression of ANP, TGF-β1, and HIF-1αcompared to their control. These findings suggest that HIF-1αand ANP, synthesized by the kidney, are involved in an adaptive mechanism in response to a sodium overload to prevent or attenuate the deleterious effects of the oxidative stress and the hypoxia on the development of fibrosis.Erratum to “Renal Overexpression of Atrial Natriuretic Peptide and Hypoxia Inducible Factor-1α as Adaptive Response to a High Salt Diet” |
| Databáze: | OpenAIRE |
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