Determining enantiomeric excess from overlapping HPLC peaks: discussion of errors in the methods
| Autor: | Albrecht Mannschreck, Miroslav Holík |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Circular dichroism
010405 organic chemistry Chemistry Process Chemistry and Technology 010401 analytical chemistry Autocorrelation Analytical chemistry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Computer Science Applications Analytical Chemistry Dual detector Range (statistics) Sensitivity (control systems) Total least squares Enantiomeric excess Spectroscopy Software |
| Zdroj: | Chemometrics and Intelligent Laboratory Systems. 72:153-160 |
| ISSN: | 0169-7439 |
| Popis: | Enantiomeric excess can be determined from overlapping liquid chromatography peaks when a dual detector, e.g., UV/vis and circular dichroism (CD), is used. Two literature methods for the determination of the sensitivity factor, g, were analyzed and the errors involved in the calculation of the enantiomeric excess evaluated. It was shown that the most important part of the task is selecting the range of data used for the calculation of g. These data should be tested for autocorrelation and the data range reselected if the autocorrelation is high. The best results are obtained when both methods are used and the deconvolved curves obtained in each case fit completely to one another. Then the sensitivity factors, g, obtained by both methods are almost the same and the errors in the enantiomeric excess determination are minimized. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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