Isolation and characterization of bacteriocin producing Enterococcus casseliflavus and its antagonistic effect on Pseudomonas aeruginosa
| Autor: | T.C. Venkateswarulu, K. Vidya Prabhakar, K. Abraham Peele, S. Krupanidhi, M. Indira |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physics and Astronomy (miscellaneous) Peptide medicine.disease_cause Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology 03 medical and health sciences 0404 agricultural biotechnology Bacteriocin Computer Science (miscellaneous) Enterococcus casseliflavus medicine chemistry.chemical_classification 030109 nutrition & dietetics Multidisciplinary Molecular mass biology Chemistry Pseudomonas aeruginosa food and beverages 04 agricultural and veterinary sciences biochemical phenomena metabolism and nutrition Antimicrobial biology.organism_classification 16S ribosomal RNA 040401 food science Chemistry (miscellaneous) bacteria Bacteria |
| Zdroj: | Karbala International Journal of Modern Science. 4:361-368 |
| ISSN: | 2405-609X |
| Popis: | The discovery of antimicrobial bacteriocin molecules with broad-spectrum activity against bacterial infections caused by spoilage microorganisms is vital for food safety. In this study, a potential bacteriocin-producing bacterium was isolated, screened and confirmed as Enterococcus casseliflavus MI001 by 16s rDNA sequencing. The bacteriocin peptide was purified and analysed by MALDI-TOF, which revealed the presence of a bacteriocin ABC transporter protein with a molecular mass of 22.53 kDa. The purified bacteriocin gave a final yield of 2.0%, with a specific activity of 15,000 AU/mg. The bacteriocin showed antagonistic activity against Pseudomonas aeruginosa and has potential as an antioxidant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|