ELECTRON MICROSCOPIC AUTORADIOGRAPHY OF THE TRANSCRIPTION ON ISOLATED CHROMATIN FIBERS

Autor:	Sachiko Omura, Takuzo Oda
Rok vydání:	1975
Předmět:	Histology Physiology Direct evidence RNA Cell Biology Biology Biochemistry In vitro Pathology and Forensic Medicine law.invention Chromatin Cell biology chemistry.chemical_compound chemistry law Transcription (biology) RNA polymerase Ultrastructure Electron microscope
Zdroj:	ACTA HISTOCHEMICA ET CYTOCHEMICA. 8:304-313
ISSN:	1347-5800 0044-5991
DOI:	10.1267/ahc.8.304
Popis:	In vitro transcription was made on chromatin fibers isolated from rat liver and several types of tumor cells using E. coli RNA polymerase and 3H-uridine triphosphate-containing substrates. Electron microscopy of the chromatin fibers after the transcription reaction revealed a characteristic structure with RNA-like fibers attached to the chromatin fibers. Electron microscopic autoradiography of these specimens demonstrated silver grains along the RNA-like fibers. This finding provided direct evidence that these fibers were real RNA molecules newly transcribed in vitro. Analysis of the ultrastructure of these transcription complexes revealed that the transcriptionally active regions of the chromatin had extended fibers bound with proteins. Long stretches of free DNA were not found in the active regions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dbf5971bdf90c08b7e80cbe1befc5cde https://doi.org/10.1267/ahc.8.304 Zobrazit plný text záznamu