Characterization of expressed sequence tags obtained by SSH during root formation in Larix cuttings

Autor: Jian Dong, Li-wang Qi, Xiao-mei Sun, Jian Feng, Shou-gong Zhang
Rok vydání: 2010
Předmět:
Zdroj: Forestry Studies in China. 12:137-141
ISSN: 1993-0372
1008-1321
DOI: 10.1007/s11632-010-0305-x
Popis: SSH was used to analyze gene transactivation during root formation of Larix cuttings. Two subtractive cDNA libraries were constructed from clone 31-6 as tester or driver and clone 15-4 as driver or tester. The SSH PCR products from the libraries were cloned into a pGEM-T easy vector and after PCR and dot blot analysis, positive clones were selected, sequenced and compared to the database in GenBank with BLASTX. The results of a sequence assembly in two libraries show that 521 UniEST (expressed sequence tag) were obtained. These 521 UniEST belong to metabolism, signal pathways, transport, resistance, developmental processes, localization, unknown proteins and “no hits found”. All of these suggest that subtractive cDNA libraries during root formation of Larix cuttings were constructed successfully.
Databáze: OpenAIRE
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