Analysis of antigen-antibody cross-reactivity among lineages and sublineages ofBabesia microtiparasites using human babesiosis specimens

Autor: Masahiro Satake, Chieko Matsumoto, Aya Zamoto-Niikura, Masayuki Saijo, Keiji Matsubayashi, Yusuke Sayama, Chiaki Ishihara, Tadashi Nagai
Rok vydání: 2018
Předmět:
Zdroj: Transfusion. 58:1234-1244
ISSN: 0041-1132
DOI: 10.1111/trf.14558
Popis: BACKGROUND Human babesiosis is caused mainly by Babesia microti and has recently become a public health concern due to an increase in transfusion-transmitted infection. Thus, the development of an antibody detection method with high specificity and sensitivity is a priority. Seroreactivity against B. microti has been reported to be highly specific not only to B. microti lineages but also to sublineages. This study aimed to elucidate the human antibody reactivity against various lineages, including US, Kobe, and Hobetsu, and sublineages (North America and East Asia) in the US lineage. STUDY DESIGN AND METHODS Twenty samples obtained from individuals infected with B. microti in the United States were tested for the presence of anti-B. microti antibodies using indirect immunofluorescence assay (IFA) and Western blotting (WB) to indicate antigens of each (sub-)lineage. RESULTS By IFA, 20 samples showed reactivity to the North America sublineage (titer range, 64-4096), 16 to the East Asia sublineage (64-512), 10 to the Kobe (64-128), and five to the Hobetsu (64). Antibody titers to the East Asia sublineage, Kobe, and Hobetsu were significantly lower than those to the North America sublineage (p
Databáze: OpenAIRE
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