TLC/HPTLC and HPLC methods for monitoring microbial transformation processes

Autor: Balázs Erdélyi, Gábor Seres, Antal Szabó, Janos Salat, József Ivanics, Attila Konya, Laszlo Birincsik
Rok vydání: 2004
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 17:132-136
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.17.2004.2.9
Popis: TLC methods have been developed for monitoring microbial enantioselective reduction and regioselective oxidation. The work discussed in this paper focused on preparation of aromatic chiral alcohols by bioreduction of their respective acetone derivatives by biocatalysis using yeast strains in medium containing added resin. A simple method and suitable device were developed for sampling from such heterogeneous fermentation media. Methods and chromatograms are presented for quantitation by TLC and for enantiomer ratio determination by HPLC. With regard to oxidation procedures, a TLC method was established for monitoring compactin hydroxylation during fermentation processes — as a representative example. Improvement of the method and use of HPTLC plates resulted in high resolution and separation capacity equal to that of the HPLC method. By means of the method by-products usually formed as minor components in the biotransformation could also be detected. Repeated clogging of the HPLC column as a result of imp...
Databáze: OpenAIRE
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