Cloning and molecular characterization of Myb transcription factors from Leymus (Poaceae: Trticeae)
| Autor: | Yonghong Zhou, Haiqin Zhang, Ying-Xia Lei, Gang Gao, Ruiwu Yang, Jian-Jun Lai, Yan Zhang, Yangyi Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cloning Genetics biology fungi Cell Biology Plant Science Leymus Molecular cloning biology.organism_classification 01 natural sciences Biochemistry 03 medical and health sciences Open reading frame 030104 developmental biology Arabidopsis thaliana Animal Science and Zoology MYB Molecular Biology Gene Transcription factor Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Biologia. 71:1119-1130 |
| ISSN: | 1336-9563 0006-3088 |
| Popis: | Plant Myb transcription factors arc crucial in regulating secondary metabolism, cellular morphogenesis and responses to hormone and stress signals. In this study, 19 Myb gene fragments were isolated from five Leymus species, three of which, LrMybSi LmMybS and LmMybG were nearly complete open reading frames encoding putative Leymus Myb genes. Sequence and phylogenetic analysis indicated that all these putative Myb from Leymus represent plant typical R2R3-Myb genes. Compared with Myb gene in Arabidopsis thaliana, the 19 Leymus Myb gene fragments of the R2R3 region amino acid showed three different types, W-x (19) W-x (19) W-x (12) F-x (18) W-x (18) W, W-x (19) W-x (19) W-x (12) M-x (18) W-x (18) W and W-x (19) W-x (19) R-x (12) L-x (18) W-x (18) W. LmMybS gene shares high similarity to ZmMyb39 which was possible involvement of caffeic acid O-methyl-transferase (COMT) in the lignification process. The LmMyb8 gene is expressed at high level in leaves and roots while at low level in stems. |
| Databáze: | OpenAIRE |
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