Disruption of Lipid Rafts Induces Gonadotropin Release in Ovine Pituitary and LbetaT2 Gonadotroph Cells1
| Autor: | Xavier Cayla, J. Cognié, F. Foulon-Gauze, E. Robin, J. Fontaine |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
0303 health sciences medicine.medical_specialty Gonadotroph Cell medicine.drug_class Cell Biology General Medicine Biology Gonadotropic cell 03 medical and health sciences Follicle-stimulating hormone 0302 clinical medicine Endocrinology Reproductive Medicine Internal medicine medicine Secretion Viability assay Gonadotropin Luteinizing hormone Lipid raft hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Biology of Reproduction. 79:17-25 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.107.064881 |
| Popis: | In order to better understand the cellular mechanisms underlying LH and FSH secretion, we have addressed the contribution of lipid rafts to the secretion of gonadotropins. We used methyl-beta-cyclodextrin (MbetaCD), a cholesterolsequestering agent, on an LbetaT2 murine gonadotroph cell line and on primary cultures of ovine pituitary cells. We found that in both systems, cholesterol depletion by MbetaCD induced a fast and substantial release of LH in the absence of natural stimulation by GnRH. In ovine pituitary cells, MbetaCDmediated LH release was shown to be independent of protein synthesis. Twenty-four hours after MbetaCD treatment, there was no loss of cell viability and full recovery of LH secretory capabilities, as determined by GnRH or MbetaCD treatment. In addition, our data suggest the existence of a pool of LH that is not released by GnRH treatment but that is released by MbetaCD treatment. Finally, in ovine pituitary cells, MbetaCD treatment induced FSH secretion. Importantly, these in vitro data are supported by in vivo studies, because MbetaCD injected into the pituitary glands of anaesthetized sheep reproducibly induced a peak of LH release. |
| Databáze: | OpenAIRE |
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