An Improved High Performance Liquid Chromatographic Method for Identification and Quantization of Polyamines as Benzoylated Derivatives

Autor:	Sajid Bashir, Rajat Sethi, Mauro E. Castro, Sai Raghuveer Chava
Rok vydání:	2011
Předmět:	Solvent Psychiatry and Mental health chemistry.chemical_compound Cadaverine Chloroform Chromatography chemistry Elution Reversed-phase chromatography Diethyl ether Derivatization Acetonitrile
Zdroj:	American Journal of Analytical Chemistry. :456-469
ISSN:	2156-8278 2156-8251
DOI:	10.4236/ajac.2011.24055
Popis:	A simple reversed phase HPLC method was developed for the determination of Putrescine, Cadaverine and Spermidine (a class of polyamines) in their benzoylated form from external known standards. In the optimization procedure, a number of parameters were examined: 1) Solvent used in the extraction of standard polyamines (diethyl ether versus chloroform); 2) Solvent used in the elution of the polyamine (methanol versus acetonitrile); 3) Mode of derivatization and extraction step(s) (derivatization and extraction performed together versus derivatization and extraction performed separately); and 4) Other instrumental parameters (such as UV detection wavelength, gradient profiles). The advantages of our method, relative to the standard Morgan method are: a) decreased chromatographic runtime, b) ease of preparation with good resolution, sensitivity, and reproducibility using a standard RP-HPLC method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dbbd0a19ffe26c7498c55880b3a0e66d https://doi.org/10.4236/ajac.2011.24055 Zobrazit plný text záznamu