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In recent years, plant-based nanoparticle synthesis and research attained significant attraction due to its potential biological application. Presently studies in bio-nanotechnology have attained fascinating attention due to the use of nanoparticles in various fields of life. We used aqueous leaf extract of Alpinia calcarata to synthesize silver nanoparticles (AgNPs) and characterized them using UV-vis spectra, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and other techniques. These AgNPs' antibacterial and anticancer properties were also investigated. SEM analysis depicted the uniformly distributed spherical nanoparticles with 20 to 22 nm size. EDX analysis revealed the presence of silver ions with a strong signal. Gram-positive and negative human pathogenic bacteria such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa were inhibited by the synthesized AgNPs. Antibacterial activities of the AgNPs were directly proportional to the concentration. The diameter of the zone of inhibition of AgNPs on bacteria varies from species to species. AgNPs were also found to have anticancer action against the MFC7 human breast cancer cell line. The anticancer activity of synthesized AgNPs was directly proportional to the concentration. The IC50 of this AgNP on the MCF7 cell line was determined using a linear regression equation and found to be 219.64 µg/ml. Thus, A. calcarata aqueous leaf extract can be used to synthesize AgNPs in an eco-friendly manner, and these AgNPs can be used in anticancer and antibacterial medicines, food, and cosmetic industries after clinical studies. |
