A role of dendritic cell from peripheral blood on mixed lymphocyte reaction

Autor:	Fumihiko Imai, Toshiko Ishibashi, Teruhiko Suzuki
Rok vydání:	1987
Předmět:	Follicular dendritic cells Cell growth T cell fungi Immunology Naive B cell hemic and immune systems chemical and pharmacologic phenomena General Medicine Dendritic cell Biology Molecular biology medicine.anatomical_structure medicine Immunology and Allergy Antigen-presenting cell B cell Histiocyte
Zdroj:	Japanese Journal of Clinical Immunology. 10:566-571
ISSN:	1349-7413 0911-4300
Popis:	Preparation of dendritic cells were obtained from a relatively small amount of human peripheral blood. These isolates contained from 30 to 60% of dendritic cells proving with the morphological characteristics of human lymphoid dendritic cell. By using monoclonal antibodies, they seemed to possess HLA-DR antigen and C3bi receptor. These preparations were compared with macrophage preparation and B cell preparation as a stimulator in autologous mixed lymphocyte reaction (auto-MLR). Auto-MLR was quantitated by the 3H-thimidine incorporation.The dendritic cell preparation provided the most potent stimulus for T cell proliferation in auto-MLR. On the other hand, macrophage preparation and B cell preparation were a little or not stimulous for auto-MLR. These data suggested that dendritic cell was major stimulator in auto-MLR.T cell proliferation in auto-MLR was reduced by adding anti-OKDR monoclonal antibody. Dendritic cell was also stimulatory cell in allogeneic MLR.Our results would be able to apply the clinical investigation of dendritic cell function in some patients with depressed auto-MLR.
Databáze:	OpenAIRE
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