In vitro antioxidant activity of macroalgae Sargassum duplicatum and Palmaria palmata extracts collected from Sepanjang Beach, Gunungkidul, Yogyakarta
Autor: | D. J. Prasetyo, C. D. Poeloengasih, N Indirayati, Cici Darsih, A. W. Indrianingsih |
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Rok vydání: | 2021 |
Předmět: | |
Zdroj: | IOP Conference Series: Materials Science and Engineering. 1011:012052 |
ISSN: | 1757-899X 1757-8981 |
DOI: | 10.1088/1757-899x/1011/1/012052 |
Popis: | Marine macroalgae are considered as important sources for bioactive phytochemicals. In this research, two potential marine macroalgae, i.e. Sargassum duplicatum and Palmaria palmata taken from Sepanjang beach, Gunungkidul, Yogyakarta, Indonesia were explored as sources of phenolic compound, and their antioxidant activities were evaluated. Three different solvents, i.e. chloroform, ethyl acetate, and methanol were applied as extraction medium. For each species, its total phenolic content (TPC) and antioxidant activity were determined using Folin-Ciocalteu reagent and DPPH scavenging assay, respectively. The result showed that methanolic extracts exhibited the highest yield in both species. The highest TPC (726.54 mg GAE/g) was obtained from S. duplicatum extracted using ethyl acetate. The antioxidant activity from S. duplicatum and P. palmata have similar value with IC50 values of 790.34 µg/mL and 789.29 µg/mL. The scavenging activities of all extracts related to the total polyphenol content, whereas the polarity of solvent affected the resulted yield and TPC of the extracts. However, our results demonstrated that all extracts were inactive against DPPH radicals (IC50 > 250 μg/mL). |
Databáze: | OpenAIRE |
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