Generation of hydrogen peroxide in protein solutions under the influence of thermal and optical electromagnetic radiation
| Autor: | Ekaterina Karmanova, Roman Davidov, Vitaly Chelnokov, Valentin Vershinin, Ivan Savchenko, Vladimir Bruskov, Valentin Ivanov |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | IOP Conference Series: Earth and Environmental Science. 390:012023 |
| ISSN: | 1755-1315 1755-1307 |
| Popis: | Using the method of induced chemiluminescence in solutions of bovine serum albumin (BSA) and bovine gamma globulin (BGG) under the influence of thermal and optical electromagnetic radiation, the formation of long-lived reactive protein species (LRPS) with a half-life of about 4-5 hours was registered. The oxygen effect was established, namely, the dependence of the chemiluminescence intensity of protein solutions on the concentration of dissolved oxygen. Using the enhanced chemiluminescence method in the luminol-p-iodophenol-peroxidase system, the ability of LRPS, induced by heat and laser radiation, to generate hydrogen peroxide in solution was studied. The dependence of the formation of H2O2 under the influence of LRPS at different times after exposure was established. Using the fluorescent probe coumarin-3-carboxylic acid, the formation of hydroxyl radicals in protein solutions after exposure to laser radiation and heat was detected. The obtained experimental data suggest that the generation of hydrogen peroxide by LRPS after exposure to thermal and optical electromagnetic radiation may be one of the mechanisms for activating protective cellular mechanisms that help overcome diseases, including those associated with oxidative stress. |
| Databáze: | OpenAIRE |
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