Attachment and Biofilm Formation of Mycobacterium marinum on a Hydrophobic Surface at the Air Interface
| Autor: | Jill Arnold, Manoucher Shahamat, Hem D. Shukla, Brent Whitaker, Mohammad R. Alavi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Polypropylene
Chromatography biology Physiology Alanine dehydrogenase Biofilm General Medicine biology.organism_classification Applied Microbiology and Biotechnology Hydrophobic effect Cell wall chemistry.chemical_compound chemistry Biophysics Microaerophile Mycobacterium marinum Bacteria Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 23 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-006-9198-1 |
| Popis: | Properties of attachment of Mycobacterium marinum to hydrophobic surfaces and subsequent␣biofilm formation were investigated. Binding of M. marinum to polypropylene occured under aerobic and anaerobic/microaerophilic conditions. However, aerobic conditions were necessary for biofilms to persist. Highly non-polar organic solvents were found to efficiently remove attached bacteria from the polypropylene surface, indicating strong hydrophobic interactions between the M. marinum cell wall and the surface. Increased capsular material, occurring during stationary phase, correlated with a decrease in attachment of cells to polypropylene. A protein of approximately 40 kDa appears to be present in increased amounts during the stationary phase. The protein has been identified by LC MS/MS analysis as alanine dehydrogenase. |
| Databáze: | OpenAIRE |
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