Maize seed germination inhibition test for pregnancy diagnosis in Zebu cows

Autor: I. A. Adebisi, H. O. Amusa, O. O. Oyinlola, O. A. Olorunnisomo, J. A. Alalade, C. B. Emiola, O. O. Okunlola
Rok vydání: 2020
Předmět:
Zdroj: Nigerian Journal of Animal Production. 46:101-107
ISSN: 0331-2062
Popis: Pregnancy diagnosis is an important part of good management and helps to maximize reproductive performance. Over time, different methods for detection of pregnancy in cows and other female domestic animals have been developed which include observation, physical examination, chemical tests and electronic instruments. Seed germination inhibition technique was carried out to detect pregnancy in Zebu cows. The urine samples collected from four pregnant cows, four non pregnant cows, four bulls and water (control) were subjected to seed germination test using maize seeds. Urine was collected between 5 and 7 am. Pregnant cows were confirmed by rectal palpation. The pH of the urine samples was determined before and after dilution and consequently, the urea concentration was also analyzed in the laboratory. The urine was diluted at the ratio of 1:4 with distilled water. Control test was also carried out with the addition of water only to the maize seeds. In each sterile Petri dish fifteen maize seeds were taken on the filter paper and 15mL of diluted urine was added. The seed germination inhibition percentages were observed after five days while shoot length growth inhibition was also observed after five days. Germination inhibition percentage of pregnant cows (64.16%) was significantly (P
Databáze: OpenAIRE