Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi

Autor:	Valentin N. Petushkov, Natalja S. Rodionova, Ilia V. Yampolsky, K. V. Purtov, T. V. Chepurnykh, A A Kotlobay, N M Myshkina, K A Beregovaya
Rok vydání:	2021
Předmět:	chemistry.chemical_classification 0303 health sciences Enzyme function Protein family Chemistry 030302 biochemistry & molecular biology Biophysics Rational design Mutagenesis (molecular biology technique) General Chemistry General Medicine Biochemistry 03 medical and health sciences Enzyme Bioluminescence Luciferase Neonothopanus nambi 030304 developmental biology
Zdroj:	Doklady Biochemistry and Biophysics. 496:14-17
ISSN:	1608-3091 1607-6729
Popis:	The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::db6bcc5f8c13209ff0f29787a497217f https://doi.org/10.1134/s1607672921010026 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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