Identification of plant-derived genetically modified organisms in food and feed using a hydrogel oligonucleotide microchip

Autor: G. A. Romanov, Alexander S. Zasedatelev, D. A. Gryadunov, I. A. Getman, V. M. Mikhailovich, S. I. Chizhova
Rok vydání: 2011
Předmět:
Zdroj: Molecular Biology. 45:894-903
ISSN: 1608-3245
0026-8933
DOI: 10.1134/s0026893311050062
Popis: A method of multiplex polymerase chain reaction (PCR) followed by hybridization on a hydrogel oligonucleotide biochip was developed for simultaneous identification of ten different transgenic elements of plant DNA in food and feed products. The biochip contained 22 immobilized oligonucleotide probes that were intended for (1) detection of plant DNA, (2) determination of plant species (soybean, maize, potato, and rice), and (3) identification of transgenic elements, including sequences of 35S CaMV, 35S FMV, rice actin gene promoters, nos, 35S CaMV, ocs, pea rbcS1 gene terminators, and bar, gus, and nptII marker genes. The limit of detection was 0.5% for genetically modified (GM) soybean and maize in the analyzed samples. The tests on food and feed products using the developed approach and real-time PCR showed full agreement in determination of transgenic DNA in the samples. The proposed assay can be used for selection of GM samples by screening food and feed products for subsequent quantitative determination of GM component based on the identified transgene.
Databáze: OpenAIRE
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