FTO gene is related to obesity in Chilean Amerindian children and impairs HOMA-IR in prepubertal girls
Autor: | Benilde Riffo, Jenny Lagos, Paulina Bustos, Claudio Aguayo, Jorge Sapunar, Isabel J Muñoz, Carlos Celis-Morales, Sylvia Asenjo, Natalia Ulloa, Katia Sáez |
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Rok vydání: | 2011 |
Předmět: |
medicine.medical_specialty
business.industry Endocrinology Diabetes and Metabolism Case-control study nutritional and metabolic diseases Odds ratio medicine.disease FTO gene Obesity Childhood obesity Endocrinology Insulin resistance Internal medicine Pediatrics Perinatology and Child Health Internal Medicine medicine business Allele frequency Body mass index |
Zdroj: | Pediatric Diabetes. 13:384-391 |
ISSN: | 1399-543X |
Popis: | Objective The objective of this study was to investigate the allelic frequency of the fat mass and obesity-associated (FTO) gene (rs9939609) and its influences on obesity and metabolic risk biomarkers in a cohort of normal weight and obese Chilean children determining its ethnicity. Methods A total of 136 normal weight children and 238 obese children (between 6 and 11 yr old) from an urban setting were recruited for this case–control study. The children were classified as normal weight [body mass index (BMI) ≥ 5th and 95th percentile), according to the international age- and gender-specific percentiles defined by the Center for Disease Control and Prevention. The analysis of serum markers was carried out using commercial kits. The FTO polymorphism was determined through a high-resolution melting enabled real time polymerase chain reaction. Ethnicity was determined by analyzing mitochondrial DNA by the restriction fragment length polymorphism method. Results As much as 85% of the cohort was Amerindian. The minor A allele of rs9939609 was associated with obesity (odds ratio (OR): 1.422 [95% confidence interval (CI) 1.068–1.868] p = 0.015), calculated using an additive model. In sex-stratified analysis we found that the risk variant (A) of rs9939609 was associated with a higher homeostasis model of assessment for insulin (HOMA-IR) in prepubertal obese girls. In male carriers of the A allele, HOMA-IR showed no further deterioration than that already associated with obesity. Conclusions In summary, we confirm the association of the FTO gene single-nucleotide polymorphism rs9939609 with obesity in Chilean Amerindian children. Furthermore we show an association between the risk allele (A) and insulin resistance-related markers in prepubertal obese girls. |
Databáze: | OpenAIRE |
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