Tumor cell detection device based on surface plasmon resonance imaging and image processing

Autor:	Maokai Yuan, Wei Jin, Yicong Lang, Bo Ouyang, Liancheng Xiang, Ying Mu, Fan Liu, Mengchao Yang, Xinyu Jin, Chao Zhou, Zhongyu Wu
Rok vydání:	2014
Předmět:	Materials science business.industry media_common.quotation_subject Image processing Tumor cells General Chemistry Chip Tumor detection Optics Hepg2 cells Surface plasmon resonance imaging Contrast (vision) Surface plasmon resonance business media_common
Zdroj:	Chemical Research in Chinese Universities. 30:211-215
ISSN:	2210-3171 1005-9040
DOI:	10.1007/s40242-014-3415-5
Popis:	A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing. In this device, a gravity-induced flow injection chip(gFIC) was exploited to replace a pump. Also two charge coupled devices(CCDs) were used to detect HepG2 cells by SPRi and image processing, respectively. The results of two CCDs are associated. Protein A was used to modify the sensing surface. The inlet angle was carefully adjusted for the device to get an enhanced image. In the test, the contrast among cell solutions at different concentrations can be easily distinguished. The other CCD using image processing can tell false-positive in some degree. This detection is label-free, real time, and precise.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::db4dc02235c63a49700b38b64a0a12a4 https://doi.org/10.1007/s40242-014-3415-5 Zobrazit plný text záznamu Full text from SpringerLink