Determination of Lamotrigine in Plasma of Infant Patients with Epilepsy Undergoing Polypharmacy by High-performance Liquid Chromatography

Autor:	Kenjiro Kikuchi, Koichi Imai, Motoyuki Minamitani, Kotoko Sugaya, Ryuki Matsuura, Manabu Tanaka, Shin-ichiro Hamano, Masumi Nakamura, Erika Nakashima, Yasutoshi Arai, Ryohei Takahashi, Terumitsu Yoshida
Rok vydání:	2011
Předmět:	Epilepsy Chromatography Correlation coefficient Chemistry Chlorzoxazone medicine Protein precipitation Gradient elution Plasma Lamotrigine medicine.disease High-performance liquid chromatography medicine.drug
Zdroj:	Iryo Yakugaku (Japanese Journal of Pharmaceutical Health Care and Sciences). 37:217-224
ISSN:	1882-1499 1346-342X
DOI:	10.5649/jjphcs.37.217
Popis:	We developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for determining lamotrigine (LTG) plasma concentrations in infant patients with epilepsy undergoing polypharmacy.It involves a simple protein precipitation procedure that uses no solid-phase or liquid-liquid extraction.HPLC was carried out on a Cadenza CD-C 18 column (3μm,4.6 mm×150 mm) with phosphate buffer (pH 4.6;25 mM) containing a methanolacetonitrile mixture (4:3,v/v) as the mobile phase,by gradient elution at a 1.0 mL/min flow rate.LTG was detected using a UV detector at 215 nm.The quantification limit was 0.10μg/mL for 100μL of plasma sample.The intra- and interassay coefficients of variation were 1.18-6.87% and 1.65-7.07%,respectively.A specificity and selectivity study showed that there were no interfering peaks.In addition,correlation analysis conducted on LTG concentrations by the new method and a commercial HPLC method produced a correlation coefficient (r ) of 0.9974.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::db49ed1d07e29934686eea27f28f8c0b https://doi.org/10.5649/jjphcs.37.217 Zobrazit plný text záznamu