Avian Influenza Virus RNA in Groundwater Wells Supplying Poultry Farms Affected by the 2015 Influenza Outbreak
| Autor: | Susan K. Spencer, Dana W. Kolpin, Laura E. Hubbard, Aaron D. Firnstahl, Joel P. Stokdyk, Mark A. Borchardt |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses animal diseases Health Toxicology and Mutagenesis 030106 microbiology Hemagglutinin (influenza) 010501 environmental sciences medicine.disease_cause 01 natural sciences Virus 03 medical and health sciences Influenza A virus medicine Environmental Chemistry Waste Management and Disposal Feces 0105 earth and related environmental sciences Water Science and Technology Ecology biology Parvovirus business.industry virus diseases Outbreak Poultry farming biology.organism_classification Pollution Virology biology.protein business Groundwater |
| Zdroj: | Environmental Science & Technology Letters. 4:268-272 |
| ISSN: | 2328-8930 |
| DOI: | 10.1021/acs.estlett.7b00128 |
| Popis: | During the 2015 outbreak of highly pathogenic avian influenza virus (HPAI) on poultry farms in the midwestern United States, concern was raised about the potential for HPAI to contaminate groundwater. Our study objective was to evaluate the occurrence of HPAI in the groundwater supply wells on 13 outbreak-affected poultry farms in Iowa and Wisconsin. We sampled 20 wells, six waste-storage lagoons, and one pond. Three wells and one lagoon were positive for the matrix gene indicative of influenza A virus. Using a semi-nested qPCR assay specific to the H5 HPAI outbreak strain, one well was H5-positive, matching the outbreak virus hemagglutinin gene. Matrix gene-positive samples analyzed for avian influenza virus (AIV) by cell culture and embryonating egg culture were negative. Seven wells were positive by PCR for a poultry-specific parvovirus, thus providing corroborating evidence of virus transport pathways between poultry fecal wastes and groundwater. Our data suggest it is possible for AIV to be transport... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|