Evaluation of commercial loop-mediated isothermal amplification based kit and ready-to-use plating system for detection of Salmonella in naturally contaminated poultry and their processing environment
| Autor: | S. R. Chandraprasad, Hafiz Nidaullah, Ahamed Kamal Shamila-Syuhada, Li-Oon Chuah, Nadarajan Abirami, Nurul Huda, Gulam Rusul, Hassim Hasmaizal |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Salmonella business.industry 030106 microbiology 010401 analytical chemistry Loop-mediated isothermal amplification Contamination Biology medicine.disease_cause 01 natural sciences Predictive value Petrifilm 0104 chemical sciences Biotechnology 03 medical and health sciences Screening method medicine Ready to use Food science business Food Science |
| Zdroj: | Food Control. 70:74-78 |
| ISSN: | 0956-7135 |
| Popis: | This current research evaluated the efficacy of two rapid methods: the 3M™ Molecular Detection Assay (MDA) and the 3M™ Petrifilm™ Salmonella Express (SALX) System against culture-based method for the detection of Salmonella in naturally contaminated poultry and their environment. One hundred forty five samples consisting of whole chickens, chicken cuts and environmental samples were analysed for the presence of Salmonella . 133 (91.7%), 124 (85.5%) and 123 (84.8%) samples were positive by the culture-based method, 3M MDA and 3M Petrifilm SALX System, respectively. The sensitivity, specificity, negative predictive value, positive predictive value and accuracy for the 3M MDA were 93.2, 91.7, 55.0, 99.2 and 93.1%, respectively. Similarly, the sensitivity, specificity, negative predictive value, positive predictive value and accuracy for the 3M Petrifilm SALX System were 92.5, 100.0, 54.5, 100.0 and 93.1%, respectively. Both 3M MDA and 3M Petrifilm SALX System yielded similar kappa results, indicating substantial agreement with the culture-based method. The data from this study therefore supports the use of the 3M Petrifilm SALX System and the 3M MDA as rapid and reliable screening methods for Salmonella detection in poultry and its processing environment. |
| Databáze: | OpenAIRE |
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