| Autor: |
Heinz Rüterjans, J. Simon, Eberhard Hoffmann, Jürgen M. Schmidt |
| Rok vydání: |
1988 |
| Předmět: |
|
| Zdroj: |
Nucleosides and Nucleotides. 7:757-761 |
| ISSN: |
0732-8311 |
| Popis: |
The solution structure of RNase T1 and its complexes with 2′-GMP and. 3′-GMP have been investigated by combined use of 2D-NMR spectroscopy and restrained molecular dynamics calculations (MD). An analysis of the nuclear Overhauser effects (NOEs) observed indicates the presence of one a helix as well as of two antiparallel β sheets. Interaction of the nucleotides with the active site leads to changes of the backbone conformation of the amino acids involved. However, the interaction between the protein and 3′-GMP is not as strong as the interaction with 2′-GMP, possibly because of weaker binding. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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