Expression profiles of sporulation genes in multidrug-resistant Bacillus species isolated from intensive care units of Ghanaian hospital
| Autor: | Abiola Isawumi, Eunice Ampadubea Ayerakwa, Molly Kukua Abban, Lydia Mosi |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Experimental Biology and Medicine. :153537022311603 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/15353702231160336 |
| Popis: | Sporulating bacteria such as Bacillus spp. have contributed to severity of opportunistic hospital acquired infections, including postoperative wounds and respiratory tract infections. This study determines the expression profiles of sporulation markers in multidrug-resistant Bacillus spp. isolated from Ghanaian hospital environments. Antimicrobial resistance (AMR) profiles of the bacteria were determined with disk diffusion and broth microdilution. Primer-specific polymerase chain reaction (PCR) amplification was used to profile the sporulation markers, and quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used for the expression of the sporulation markers at different antibiotic concentrations. The strains are multidrug resistant (70–100%) to at least two of the eight classes of the antibiotics tested including cephalosporins, penicillin, aminoglycosides, and glycopeptide. The strains showed different resistance patterns to all the tested antibiotics, which might indicate diverse resistance mechanisms. Common ( spoVK spoVE, spoJ, and sigF) and not commonly ( sigJ, soJ, yrbC, and yjcE) reported sporulation markers were detected in the strains. The study showed an association of the sporulation markers with AMR as indicated by their expression profiles. |
| Databáze: | OpenAIRE |
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