Characterization and evaluation of cytotoxicity of biphasic calcium phosphate synthesized by a solid state reaction route
| Autor: | Emiliano Barreto, Geovana Dresch Webler, J.M. Hickmann, Laís C. Agra, Maximiliano J. M. Zapata, Antonio O. S. Silva, Eduardo J. S. Fonseca |
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| Rok vydání: | 2014 |
| Předmět: |
Scanning electron microscope
Solid-state reaction route General Physics and Astronomy chemistry.chemical_element Mineralogy Calcium symbols.namesake stomatognathic system chemistry Phase (matter) visual_art visual_art.visual_art_medium symbols General Materials Science Ceramic Fourier transform infrared spectroscopy Cytotoxicity Raman spectroscopy Nuclear chemistry |
| Zdroj: | Current Applied Physics. 14:876-880 |
| ISSN: | 1567-1739 |
| DOI: | 10.1016/j.cap.2014.03.026 |
| Popis: | Calcium phosphate compounds have been widely studied for biomedical applications because of their chemical and structural similarity to the mineral phase constituting bone and teeth. In this work, biphasic calcium phosphate ceramics (HAP/β-TCP), with tunable phase composition ratio, were synthesized by a solid state reaction process. The effect of varying the heat treatment temperature (700, 800, 900, 1000, and 1100 °C) on the formation of the ceramic materials and their related cytotoxicity were examined. The phase composition and morphology of the prepared ceramic powders were characterized by X-ray diffraction and scanning electron microscopy, and the functional groups were analyzed using Fourier transform infrared spectroscopy and Raman spectroscopy. Cell culture experiments, using murine macrophages, showed that the synthesized HAP/β-TCP materials did not exhibit cytotoxicity regardless of the doses assayed or the differences in composition ratio of HAP/β-TCP, suggesting the potential of HAP/β-TCP for biological applications. |
| Databáze: | OpenAIRE |
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