| Autor: |
Olga L.T. Machado, Patricia D Ribeiro, Ronaldo S.M. Borges, Paulo R.R. Costa, Elias W. Alves |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Protein & Peptide Letters. 5:251-256 |
| ISSN: |
0929-8665 |
| Popis: |
Abstract: The present study explores conditions to activate the carboxyl group before the coupling of C-terrninus-AA with TMS-ITC. N-tert-butoxy-carbonyl amino acids (Boc-AAs) and Leucine Enkephalin were used as models and isobutyl chloroformate was used as a C-terminal activator. The reaction coupling activated AA with TMS ITC was temperature dependent. We also·report here a new, efficient, economical and ecological technique to separate the mixture of amino acids thiohydantoins (TH-AA). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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