?-Xylanase produced by Aureobasidium pullulans CBS 58475
| Autor: | C. C. Emeis, J. Dobberstein |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Chromatography
biology Chemistry Substrate (chemistry) Endoenzyme General Medicine Xylose biology.organism_classification Applied Microbiology and Biotechnology Xylan Aureobasidium pullulans Gel permeation chromatography chemistry.chemical_compound Xylanase biology.protein Maceration (wine) Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 32 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/bf00184971 |
| Popis: | Aureobasidium pullulans CBS 58475 produced β-xylanase with an activity of 5 units/ml culture filtrate. Xylose, xylan and complex substrates containing xylose served as strong inducers. Purification of the enzyme was achieved by two sets of gel permeation chromatography. The enzyme has an pH optimum at 4.25 and a temperature optimum at 60°C. At slightly acid pH and temperatures up to 60°C β-xylanase showed good stability. The analysis of cleavage products classified the β-xylanase as an endoenzyme. Together with an endopolygalacturonase, the β-xylanase enhanced the maceration of carrots compared to endopolygalacturonase alone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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