Secretin potentiates guinea pig pancreatic response to cholecystokinin by a cholinergic mechanism
| Autor: | Soledad Alcón, Maria J. Pozo, José A. Pariente, García Lj, Juan A. Rosado, Ginés M. Salido |
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| Rok vydání: | 1996 |
| Předmět: |
Pharmacology
Pancreatic juice secretion medicine.medical_specialty Physiology Trypsinogen General Medicine Secretin family Biology digestive system Secretin chemistry.chemical_compound medicine.anatomical_structure Endocrinology Gastrointestinal hormone chemistry Physiology (medical) Internal medicine Pancreatic juice medicine Pancreas hormones hormone substitutes and hormone antagonists Cholecystokinin |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 74:1342-1350 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y96-149 |
| Popis: | The effects of secretin and cholecystokinin on exocrine pancreas secretion in the guinea pig were investigated. The putative potentiating effect of these two hormones was studied in various settings to elucidate the effect of cholinergic stimuli in such interaction. In anesthetized guinea pig, intravenous infusion of cholecystokinin (0.75 pmol.kg-1.min-1) or secretin (0.5 pmol.kg-1.min-1) resulted in a marked and rapid increase of pancreatic juice flow and protein output. When cholecystokinin was combined with secretin, there was a significant increase in pancreatic, compared with cholecystokinin alone. This increase in pancreatic juice secretion and protein output was significantly suppressed by the prior administration of 100 micrograms/kg atropine. Similar results were obtained when trypsinogen release from pancreatic segments was measured in response to cholecystokinin (32 nM-32 pM) and (or) secretin (1 microM-32 nM). When we assayed the hormonal interaction on amylase release from dispersed pancreatic acini, we found that secretin (32 nM) failed to influence the secretory response to cholecystokinin (1 pM-10 nM). Thus we conclude that a combination of cholecystokinin and secretin resulted in a marked potentiation of the secretory responses in the exocrine guinea pig pancreas by a mechanism that involves cholinergic interactions present at the tissue level but not at the dispersed secretory cell level. |
| Databáze: | OpenAIRE |
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