Evidence for DNA Polymerase-alpha and -beta Activity in Sugar Beet

Autor:	Valgene L. Dunham, John M. Tymonko
Rok vydání:	1977
Předmět:	Differential centrifugation Ethanol biology Physiology Lability DNA polymerase Elution Cell Biology Plant Science General Medicine biology.organism_classification chemistry.chemical_compound chemistry Biochemistry Genetics biology.protein Glycerol Sugar beet Polymerase
Zdroj:	Physiologia Plantarum. 40:27-30
ISSN:	1399-3054 0031-9317
DOI:	10.1111/j.1399-3054.1977.tb01487.x
Popis:	DNA polymerase preparations from incubated sugar beet (Beta vulgaris) root tissue exhibited multiple activities following DEAE-cellulose chromatography and glycerol density gradient centrifugation (600-fold purification). The two major polymerase activities eluted from DEAE-cellulose differed in heat lability, pH and Mg2+ optima, and in response to ethanol, p-hydroxymercuribenzoate, and N-ethylmaleimide. Further purification by glycerol gradient centrifugation indicated the presence of a low molecular weight molecule (3.4 S), heterogeneous larger molecules (6.2, 6.8 S), and apparent aggregates (11–11.5 S). This investigation indicates similarities between sugar beet polymerases and the small (β) and large (α) molecular weight polymerases from mammalian systems.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::daee5871d807e127258c7d71d4f9d158 https://doi.org/10.1111/j.1399-3054.1977.tb01487.x Zobrazit plný text záznamu Plný text