Process Optimisation for Increased Polysaccharide Yield ofNeisseria Meningitidis(Serogroup W135) by Submerged Fermentation
| Autor: | Panpan Liu, Xiangke Xu, Chunmei Gong, Kangkang Guo, Pengbo Ning, Yanming Zhang, Chengfeng Huang, Juan Bai, Ligeng Ma |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Neisseria meningitidis serogroup W135 biology Neisseria meningitidis medicine.disease_cause biology.organism_classification Polysaccharide Submerged fermentation Microbiology chemistry Yield (chemistry) medicine Fermentation Food science Incubation Bacteria Biotechnology |
| Zdroj: | Biotechnology & Biotechnological Equipment. 26:3224-3230 |
| ISSN: | 1314-3530 1310-2818 |
| DOI: | 10.5504/bbeq.2012.0077 |
| Popis: | Serogroup W135 polysaccharides from Neisseria meningitidis constitute a vaccine antigen against the disease caused by this bacterium. In the present study, a series of pilot scale experiments were carried out in a 300 L fermentor to enhance the final polysaccharide (PS) yield for industrial vaccine production. A three-step submerged fermentation technique significantly increased the production of Neisseria meningitidis serogroup W135 polysaccharides. Optimal conditions for polysaccharide production were as follows: ammonia nitrogen of 1.0 mg/mL, culture temperature of 36.5 °C, initial incubation concentration of 5×10 cfu-mL−1 and pH 7.0. The natural slow pH recovery of half an hour was considered the culture endpoint for the submerged fermentation, which provided a balance between polysaccharide quality and quantity. The highest PS yield reached 55 μg/mL, and the obtained W135 polysaccharide satisfied the WHO criteria for suitable antigens for industrialized vaccine production by the strategies. T... |
| Databáze: | OpenAIRE |
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